02.27.12

Softening Switchgrass

How a "juvenile" maize gene can help turn prairie grass into fuel.

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Corn stalks in a cornfield Photo courtesy of USDA/ARS

Introducing a maize gene into switchgrass substantially boosted the potential of the switchgrass biomass as an advanced biofuel feedstock.

Many experts believe that advanced biofuels made from cellulosic biomass are the most promising alternative to petroleum-based liquid fuels for a renewable, clean, green, domestic source of transportation energy. Nature, however, does not make it easy. Unlike the starch sugars in grains, the complex polysaccharides in the cellulose of plant cell walls are locked within a tough woody material called lignin. For advanced biofuels to be economically competitive, scientists must find inexpensive ways to release these polysaccharides from their bindings and reduce them to fermentable sugars that can be synthesized into fuels.

An important step towards achieving this goal has been taken by researchers with the U.S. Department of Energy (DOE)'s Joint BioEnergy Institute (JBEI), a DOE Bioenergy Research Center led by the Lawrence Berkeley National Laboratory (Berkeley Lab). JBEI is one of three DOE Bioenergy Research Centers established by the DOE Office of Science’s (SC’s) Office of Biological and Environmental Research in 2007.

A team of JBEI researchers, working with researchers at the U.S. Department of Agriculture's Agricultural Research Service (ARS), has demonstrated that introducing a maize (corn) gene into switchgrass, a highly touted potential feedstock for advanced biofuels, more than doubles (250 percent) the amount of starch in the plant's cell walls and makes it much easier to extract polysaccharides and convert them into fermentable sugars. The gene, a variant of the maize gene known as Corngrass1 (Cg1), holds the switchgrass in the juvenile phase of development, preventing it from advancing to the adult phase.

"We show that Cg1 switchgrass biomass is easier for enzymes to break down and also releases more glucose during saccharification," says Blake Simmons, a chemical engineer who heads JBEI's Deconstruction Division and was one of the principal investigators for this research. "Cg1 switchgrass contains decreased amounts of lignin and increased levels of glucose and other sugars compared with wild switchgrass, which enhances the plant's potential as a feedstock for advanced biofuels."

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JBEI researchers studying Cg1 switchgrass included (foreground) Seema Singh, (from left) Chenlin Li, Lan Sun, Blake Simmons and Dean Dibble. Photo by Roy Kaltschmidt, Berkeley Lab

JBEI researchers studying Cg1 switchgrass included (foreground) Seema Singh, (from left) Chenlin Li, Lan Sun, Blake Simmons and Dean Dibble.

The research was reported in Proceedings of the National Academy of Sciences.

Lignocellulosic biomass is the most abundant organic material on earth. Studies have consistently shown that biofuels derived from lignocellulosic biomass could be produced in the United States in a sustainable fashion and could replace today's gasoline, diesel, and jet fuels on a gallon-for-gallon basis. Unlike ethanol made from grains, such fuels could be used in today's engines and infrastructures and would be nearly carbon-neutral, meaning the use of these fuels would not add significantly to net greenhouse gas emissions.

Among potential crop feedstocks for advanced biofuels, switchgrass offers a number of advantages. As a perennial grass that is both salt- and drought-tolerant, switchgrass can flourish on marginal cropland, does not compete with food crops, and requires little fertilization. A key to its use in biofuels is making it more digestible to fermentation microbes.

"The original Cg1 was isolated in maize about 80 years ago. We cloned the gene in 2007 and engineered it into other plants, including switchgrass, so that these plants would replicate what was found in maize," says George Chuck, lead author of the PNAS paper, whose research is supported by SC’s Office of Basic Energy Sciences. Chuck is a plant molecular geneticist who holds joint appointments at the Plant Gene Expression Center with ARS and the University of California, Berkeley. "The natural function of Cg1 is to hold plants in the juvenile phase of development for a short time to induce more branching. Our Cg1 variant is special because it is always turned on, which means the plants always think they are juveniles."

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George Chuck and Sarah Hake, at the Plant Gene Expression Center, in a greenhouse, kneeling down, holding switchgrass Photo courtesy of USDA/ARS

George Chuck and Sarah Hake, at the Plant Gene Expression Center, Albany, California, introduced a variant corngrass gene into switchgrass.

Chuck and his colleague Sarah Hake, another co-author of the PNAS paper and director of the Plant Gene Expression Center, proposed that since juvenile biomass is less lignified, it should be easier to break down into fermentable sugars. Also, since juvenile plants don't make seed, more starch should be available for making biofuels. To test this hypothesis, they collaborated with Simmons and his colleagues at JBEI to determine the impact of introducing the Cg1 gene into switchgrass.

In addition to reducing the lignin and boosting the amount of starch in the switchgrass, the introduction and overexpression of the maize Cg1 gene also prevented the switchgrass from flowering even after more than two years of growth, an unexpected but advantageous result.

"The lack of flowering limits the risk of the genetically modified switchgrass from spreading genes into the wild population," says Chuck.

The results of this research offer a promising new approach for the improvement of dedicated bioenergy crops, but there are questions to be answered. For example, the Cg1 switchgrass biomass still required a pre-treatment to efficiently liberate fermentable sugars.

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Two types of switchgrass displayed side by side in pots. One set is more dense and the other set is taller. Photo courtesy of USDA/ARS

Overxpression of the Cg1 gene in switchgrass (left) compared to Wild-type of switchgrass of the same age and grown under the same conditions.

"The alteration of the switchgrass does allow us to use less energy in our pre-treatments to achieve high sugar yields as compared to the energy required to convert the wild type plants," Simmons says. "The results of this research set the stage for an expanded suite of pretreatment and saccharification approaches at JBEI and elsewhere that will be used to generate hydrolysates for characterization and fuel production."

Another question to be answered pertains to the mechanism by which Cg1 is able to keep switchgrass and other plants in the juvenile phase.

"We know that Cg1 is controlling an entire family of transcription factor genes," Chuck says, "but we have no idea how these genes function in the context of plant aging. It will probably take a few years to figure this out."

—Lynn Yarris, Lawrence Berkeley National Laboratory, LCYarris@lbl.gov

Funding

DOE Office of Science, Office of Basic Energy Sciences; DOE Office of Science, Office of Biological and Environmental Research; and U.S. Department of Agriculture, Binational Agricultural Research and Development Grant

Publication

George S. Chuck, Christian Tobias, Lan Sun, Florian Kraemer, Chenlin Li, Dean Dibble, Rohit Arora, Jennifer N. Bragg, John P. Vogel, Seema Singh, Blake A. Simmons, Markus Pauly, and Sarah Hake, "Overexpression of the maize Corngrass1 microRNA prevents flowering, improves digestibility, and increases starch content of switchgrass," Proceedings of the National Academy of Sciences 108, 17550 (2011).

Related Links

DOE Joint BioEnergy InstituteExternal link

USDA Plant Gene Expression CenterExternal link

DOE Bioenergy Research Centers, Biological Systems Science Division, Office of Biological and Environmental Research, DOE Office of Science

Photochemistry and Biochemistry Research Area, Chemical Sciences, Geosciences, & Biosciences Division, Office of Basic Energy Sciences, DOE Office of Science

Last modified: 3/18/2013 11:04:48 AM