09.07.17

First Look at a Living Cell Membrane

Neutrons provide the solution to nanoscale examination of living cell membrane and confirm the existence of lipid rafts.

Click to enlarge photo. Enlarge Photo

Neutron scattering is a valuable technique for studying cell membranes, but signals from the cell’s other components such as proteins, RNA, DNA and carbohydrates can get in the way (left). A team from Oak Ridge National Laboratory made these other components practically invisible to neutrons by combining specific levels of heavy hydrogen (deuterium) with normal hydrogen within the cell (right).

The Science

The cell membrane, a thin bilayer of lipid molecules with embedded proteins, protects the cell from its outside environment and controls the movement of substances in and out of the cell. However, much about this thin bilayer of lipid molecules has remained a mystery despite extensive studies. This has been due to the difficulty of viewing a living cell membrane; previous methods employed to investigate membrane structure, such as X-rays and electron beams, damaged the membranes. Using cold neutrons  — for the first time — researchers directly examined a living cell membrane at the nanoscale.

The Impact

Using isotopes to create internal contrast in living cells, scientists determined the membrane structure and thickness of the bacterium Bacillus subtilis. The team also confirmed the existence of the long hypothesized lipid rafts. These tightly packed free-floating membrane lipids and proteins are thought to be vital to cell signaling. The rafts are also thought to facilitate the movement of essential biomolecules in and out of the cell, along with other functions. The methods developed may prove valuable in biomass feedstock and biofuel production, where bacteria have an important role.

Summary

Examining a living cellular membrane has remained an unsolved challenge up to this point due to the dynamic, chemically diverse, and fragile nature of living cells. Too small to be seen by a traditional optical microscope, neutrons emerged as the solution to studying a living lipid bilayer at the nanoscale without damaging the cell. Neutrons can be used as a probe for characterizing biological materials because a neutron beam scattered by a biological sample creates a pattern that depends on the material’s isotopic composition and reflects the material’s structural arrangement. Deuterium is an isotope of a highly abundant atom in biological matter, hydrogen. It contains a neutron and a proton, in contrast to hydrogen, which contains a single proton but no neutron. This seemingly small difference makes substituting deuterium for hydrogen an ideal approach to studying membranes and other nanoscale biological systems. Cells perceive little difference between hydrogen and its isotope, deuterium, while the isotopes appear very differently using the neutron scattering technique. A team of researchers at Oak Ridge National Laboratory (ORNL) introduced enough deuterium into the membrane of the bacterium B. subtilis to differentiate it from other cell components. Further, the team tuned the specific proportions of deuterium and hydrogen by introducing — into the cell — two fatty acid (the molecules that comprise the membrane lipids) types, with unique isotope ratios. The cell incorporated the specific mix of isotope-labeled fatty acids into its membrane, and a non-uniform distribution of lipids was observed, confirming the lipid raft hypothesis. These experiments answer some of biology’s longest-standing questions, aligning with the U.S. Department of Energy’s Office of Science mission of providing fundamental science research to address some of the most pressing challenges of our time.

Contact

PM Contact
Amy Swain, Ph.D.
Program Manager
Biological Systems Sciences Division
Office of Biological and Environmental Research
Office of Science
U.S. Department of Energy
Amy.Swain@science.doe.gov

PI Contact
John Katsaras
katsarasj@ornl.gov

Funding

This research was sponsored by the Laboratory Directed Research and Development Program (grant 6988) of Oak Ridge National Laboratory (ORNL), managed by UT-Battelle, LLC, for the U.S. Department of Energy (DOE) under contract DE-AC05-00OR22725. Support for J.K. was provided by the DOE Office of Science, Basic Energy Sciences, Scientific User Facilities Division and for R.F.S by the DOE Office of Science, Biological and Environmental Research (grant ERKP-851). This research used resources at the Oak Ridge Leadership Computing Facility at ORNL, supported by the DOE Office of Science, Office of Advanced Scientific Computing Research, Facilities Division. Small-angle neutron scattering was performed at ORNL using the Bio-SANS instrument at the High Flux Isotope Reactor, supported by the DOE Office of Science, Office of Biological and Environmental Research, Biological Systems Science Division, through the ORNL Center for Structural Molecular Biology, and the Extended Q-Range Small-Angle Neutron Scattering Diffractometer at the Spallation Neutron Source, supported by the DOE Office of Science, Basic Energy Sciences, Scientific User Facilities Division (grant ERKP-SNX).

Publications

J.D. Nickels, S. Chatterjee, C.B. Stanley, S. Qian, X. Cheng, D.A.A. Myles, R.F. Standaert, J.G. Elkins, and J. Katsaras, “The in vivo structure of biological membranes and evidence for lipid domainsExternal link.” PLoS Biology 5, 15 (2017). [DOI: 10.1371/journal.pbio.2002214]

Related Links

Oak Ridge National Laboratory press release: Neutrons provide the first nanoscale look at a living cell membraneExternal link

Highlight Categories

Program: ASCR, BER, BSSD

Performer/Facility: DOE Laboratory, SC User Facilities, ASCR User Facilities, OLCF, BES User Facilities, HFIR, SNS

Last modified: 9/12/2017 2:45:34 PM